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Mouse EGF ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 3 pg/ml
Range: 3.28 pg/ml – 800 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Mouse

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 3 pg/ml Range 3.28 pg/ml - 800 pg/ml Recovery 96 % Sample specific recovery Sample type Average % Range Cell culture supernatant 112.4 83% - 107% Serum 88.33 75% - 101% Plasma 90.23 80% - 101% Assay duration Multiple steps standard assay Species reactivity Reacts with: Mouse Product overview Abcam’s EGF (Epidermal growth factor) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse EGF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse EGF coated on a 96- well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Platform Microplate

SKU: ab100679-1x 96 test Category: Tag:

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