Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 4 pg/ml
Range
3.28 pg/ml - 800 pg/ml
Recovery
100 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 96.65 89% - 102%
Serum 103.1 93% - 112%
Plasma 113.1 79% - 137%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Abcam’s Eotaxin-2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Eotaxin-2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Eotaxin-2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Eotaxin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Eotaxin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
Elisa Kits
Mouse Eotaxin-2 ELISA Kit (CCL24)- 96 Tests
Availability:
Available on backorder
Expiry Date
Key features and details
Sensitivity: 4 pg/ml
Range: 3.28 pg/ml – 800 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Mouse
EGP 19,488.00
Available on backorder
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