Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma, Tissue Lysate
Assay type
Sandwich (quantitative)
Sensitivity
< 4 pg/ml
Range
2.74 pg/ml - 2000 pg/ml
Recovery
98 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 86.4 72% - 99%
Serum 101.2 85% - 117%
Plasma 106.6 85% - 114%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Abcam’s IGF1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IGF1 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
Elisa Kits
Mouse IGF1 ELISA Kit- 96 Tests
Availability:
Available on backorder
Expiry Date
Key features and details
Sensitivity: 4 pg/ml
Range: 2.74 pg/ml – 2000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum, Tissue Lysate
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Mouse
EGP 19,488.00
Available on backorder
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