Mouse IL-1 alpha ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 10 pg/ml
Range: 12.23 pg/ml – 3000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Mouse

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml Range 12.23 pg/ml - 3000 pg/ml Recovery > 100 %

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 113.6 104% – 123%
Serum 122.4 113% – 132%
Plasma 112.3 77% – 140%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Abcam’s IL-1 alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-1 alpha in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Platform
Microplate

SKU: ab113344-1x 96 test Category: Tag:

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