Mouse IL-1 beta ELISA kit- 96 Tests

Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 6.7 pg/ml
Range
0.016 ng/ml – 0.5 ng/ml
Recovery
98 %

Assay time
5h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Abcam’s IL-1 beta (Interleukin-1 beta) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse IL-1 beta concentrations in plasma, serum and cell culture supernatants.

An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.

Get better reproducibility in only 90 minutes with Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate