Mouse IL-5 ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 1 pg/ml Range 2.05 pg/ml - 500 pg/ml Recovery 94 % Sample specific recovery Sample type Average % Range Cell culture supernatant 95.76 85% - 104% Serum 93.44 82% - 102% Plasma 95.49 93% - 104% Assay duration Multiple steps standard assay Species reactivity Reacts with: Mouse Product overview Abcam’s IL-5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get better reproducibility in only 90 minutes with Mouse IL-5 ELISA Kit (ab204523) from our SimpleStep ELISA® range. Platform Microplate