Mouse IP10 ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 15 pg/ml Range 8.2 pg/ml - 2000 pg/ml Recovery 97 % Sample specific recovery Sample type Average % Range Cell culture supernatant 98.86 88% - 107% Serum 97.68 86% - 106% Plasma 96.42 85% - 104% Assay duration Multiple steps standard assay Species reactivity Reacts with: Mouse Product overview Abcam’s IP10 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IP10 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse IP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and IP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get results in 90 minutes with Mouse IP-10 ELISA Kit (CXCL10) (ab214563) from our SimpleStep ELISA® range. Platform Microplate