Mouse Thrombopoietin ELISA Kit- 96 Tests

Product name
Mouse Thrombopoietin ELISA Kit
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 30 pg/ml Range 27.4 pg/ml - 20000 pg/ml Recovery 94 % Sample specific recovery Sample type Average % Range Cell culture supernatant 96.23 83% - 103% Serum 93.45 82% - 103% Plasma 94.28 84% - 104% Assay duration Multiple steps standard assay Species reactivity Reacts with: Mouse Product overview Abcam’s Thrombopoietin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Thrombopoietin in serum, plasma and cell culture supernates. This assay employs an antibody specific for Mouse Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Platform Microplate