Pig IL-1 beta ELISA Kit- 96 Tests
Available on backorder
Expiry Date
Key features and details
Sensitivity: 6 pg/ml
Range: 8.23 pg/ml – 6000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Pig
EGP 20,160.00
Available on backorder
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 6 pg/ml
Range
8.23 pg/ml - 6000 pg/ml
Recovery
90 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 96.91 89% - 104%
Serum 97.32 88% - 105%
Plasma 87.75 80% - 92%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Pig
Product overview
Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate+
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