Rat Fibrinogen ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 3 ng/ml
Range: 3.125 ng/ml – 200 ng/ml
Sample type: Cell culture supernatant, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Urine
Assay type
Sandwich (quantitative)
Sensitivity
= 3 ng/ml
Range
3.125 ng/ml – 200 ng/ml
Recovery
96 %
Assay time
3h 30m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s Fibrinogen Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen in cell culture supernatant, and urine samples.
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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