Rat IL-13 ELISA Kit- 96 Tests

Detection method
Colorimetric
Precision
Intra-assay
Sample n Mean SD CV%
Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% Sample type Cell culture extracts, Tissue Extracts Assay type Sandwich (quantitative) Sensitivity < 20 pg/ml Range 27.43 pg/ml - 20000 pg/ml Recovery 114 % Sample specific recovery Sample type Average % Range Cell culture extracts 120.4 80% - 139% Tissue Extracts 107.8 81% - 135% Assay duration Multiple steps standard assay Species reactivity Reacts with: Rat Product overview Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate. This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances. It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. Platform Microplate