Rat IL-2 ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 0.1 ng/ml Range 0.12 ng/ml - 30 ng/ml Recovery 85 % Sample specific recovery Sample type Average % Range Cell culture supernatant 78.52 67% - 89% Plasma 80.35 70% - 91% Assay duration Multiple steps standard assay Species reactivity Reacts with: Rat Product overview Abcam’s IL-2 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2 in plasma and cell culture supernatants. (Rat IL-2 concentration is pretty low in normal plasma, it may not be detected in this assay). This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. We have not been able to detect the endogenous Rat IL-2 in normal serum with ab100769, only in serum spiked with Rat IL-2. Platform Microplate