Rat IL-4 ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 1.5 pg/ml
Range: 0.66 pg/ml – 160 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat
EGP 19,488.00
Available on backorder
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 1.5 pg/ml
Range
0.66 pg/ml - 160 pg/ml
Recovery
96 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 97.69 85% - 104%
Serum 96.79 84% - 103%
Plasma 94.86 82% - 102%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s IL-4 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for rat IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
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