Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 30 pg/ml
Range
10.97 pg/ml - 8000 pg/ml
Recovery
84 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 75.43 68% - 89%
Serum 92.32 83% - 102%
Plasma 84.25 75% - 94%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Rat
Product overview
Abcam’s Leptin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
Microplate
Elisa Kits
Rat Leptin ELISA Kit- 96 Tests
Availability:
Available on backorder
Expiry Date
Key features and details
Sensitivity: 30 pg/ml
Range: 10.97 pg/ml – 8000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Rat
EGP 19,488.00
Available on backorder
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